Goal: Understood the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic， is familiar with with PT-PCR analyzes the Method: TRβ△ the gene has the expression in many kinds of organizations， in which liver， the brain， the skeleton muscle， the heart， the kidney expression are Withdraws big mouse many place organizations mRNA， withdraws mRNA is the template counter duplication synthesizes the cDNA first And expands take it as template PCR increases the goal gene which TRβ△ the double strand cDNA basis copies to design two to direct the Uses fluorescence quota PCR the method to carry on the quantitative Finally: The extraction organizes total RNA， OD260/OD280 to be situated between between 7-0， OD260/230 is situated between between 5- The agarose gel electrophoresis appraises PCR to expand increases the result to demonstrate that， has the goal about anticipated 160bp to expand approximately increases the product belt TRβΔ PCR product the agarose gel electrophoresis appraisal result to indicate， expands the DNA molecular weight size after PCR which increases is the 176bp goal The goal gene and the steward gene quantitative determination， it expands increases the curve and the curve of solubility has not seen the mixed peak， simultaneously melts the tune prominent peak correspondence Tm value with to expand increases the product theory Tm to be Conclusion: The findings showed TRβ△ the gene has certain spatial distribution characteristic in the mRNA level expression: The liver， the brain， the spleen， in the kidney organization has this gene expression， also in the spleen expression quantity is higher than other organizations
