Abstract Objective : Workers peripheral blood lymphocytes of p53 and DNA damage and repair gene mRNA expression levels of benzene exposure and the environment， explore specificity， sensitivity， stability， easy to collect specimens of the advantages of detecting indicators used benzene health care professional Methods : SYBR GreenI chimeric fluorescence quantitative real-time fluorescence RT-PCR analysis， testing workers exposed to 72 (under different posts divided into Group A， Group B) and 29 staff were peripheral blood lymphocytes and related gene p53 mRNA expression Application of differential expression analysis software REST 2005， compared with the control group operating multiple groups to express differences; While peripheral blood leukocytes， hemoglobin and platelet count; Sampling of raw materials toluene， benzene spraying agent content; Workplace air toluene concentration Results : Group A and Group B p53， p21， wild， Ape1 and Mdm2 expression levels compared with the control group was no significant difference， in Group A sword， Bcl-2， Bax， and Xpc Xpa expression level downward， Group B expression edged down compared with the control group was statistically significant A group of white blood cell， hemoglobin and platelet lower than the control group， Group B platelet lower than the control group differences are Conclusion : benzene-exposed workers part of the blood cells with DNA damage and repair gene mRNA expression levels of benzene and non-exposed control group SYBR GreenI chimeric fluorescent real-time RT-PCR and REST 2005 statistical software for detection and analysis of differentially expressed mRNA level， applies to occupational population of molecular epidemiological studies and health
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