Separation of normal protein purification is a meticulous work， sometimesthe preparation of a high purity protein to pay the arduous and long-term Preparation involves physics， chemistry and biology and other wide range of knowledge， need many complicated technical operations in this complicated So far， there is no method a single or set of ready totake any kind of protein extracted from mixed protein complex， but for any kind of protein are likely to choose a proper separation procedures to obtain high purity So， the purification of a protein， a variety ofmethods to try to carry out investigation and Fully before the experiment， their desire for separation and purification of proteins have a certain understanding， and to establish a method for the analysis and identification of the corresponding， separation and purification of the smooth progress of the work to 正蛋白质的分离纯化是一件细致的工作、有时制备一个纯度较高的蛋白质要付出艰巨而长期的努力制备工作涉及物理学、化学和生物学等较广的知识，在这个繁杂的过程中需要许多复杂的技术操作到目前为止，还没有一个单独的或一套现成的方法能够把任何一种蛋白质从复杂的混合蛋白质中提取出来，但是对于任何一种蛋白质都有可能选择一套适当的分离程序以获得高纯度的制品所以，提纯某种蛋白质时，各种方法都要尝试实验前要进行充分的调查研究，对自己欲分离提纯的蛋白质有一定的了解，同时要建立相应的分析鉴定方法，以指导分离纯化工作的顺利进行。
