The Arabidopsis NPR1/NIM1 gene is a key regulator of systemic acquired resistance (SAR) Overexpression of NPR1 leads to enhanced resistance in A To investigate the role of NPR1 in monocots， we over-expressed the Arabidopsis NPR1 in rice and challenged the transgenic plants with Xanthomonas oryzae oryzae (Xoo)， the rice bacterial blight The transgenic plants displayed enhanced resistance to X RNA blot hybridization indicates that enhanced resistance requires expression of NPR1 mRNA above a threshold level in To identify components mediating the resistance controlled by NPR1， we used NPR1 as bait in a yeast two-hybrid We isolated four cDNA clones encoding rice NPR1 interactors (named rTGA1， rTGA2， rTGA3 and rLG2) belonging to the bZIP rTGA1， rTGA2 and rTGA3 share 75， 76 and 78% identity with Arabidopsis TGA2， In contrast， rLG2 shares highest identity (81%) to the maize liguleless (LG2) gene product， which is involved in establishing the leaf blade±sheath The interaction of NPR1 with the rice bZIP proteins in yeast was impaired by the npr1-1 and npr1-2 mutations， but not by the nim1-4 The NPR1±rTGA1 interaction was con®rmed by an in vitro pull-down In gel mobility shift assays， rTGA1 binds to the rice RCH10 promoter and to a cis-element required sequencespeci ®cally for salicylic acid This is the ®rst demonstration that the Arabidopsis NPR1 gene can enhance disease resistance in a monocot These results also suggest that monocot and dicot plants share a conserved signal transduction pathway controlling NPR1-mediated Arabidopsis NPR1/NIM1基因是一个关键管理者系统获取的抵抗(SAR)。 过分表现 of NPR1导致在Arabidopsis的改进的抵抗。 调查NPR1的角色 monocots，我们用米在表达了Arabidopsis NPR1并且向有的转基因植物挑战Xanthomonas稻属pv。 稻属(Xoo)，米细菌凋枯病病原生物。 转基因植物 displayed提高了对Xoo的抵抗。 核糖核酸污点杂交表明那改进的抵抗NPR1 mRNA requires表示在阈电平之上的在米。 辨认斡旋的组分NPR1控制的the抵抗，我们使用了NPR1作为诱饵在酵母二杂种屏幕。 我们隔绝了四 cDNA克隆内码米NPR1 interactors (名为rTGA1、rTGA2、rTGA3和rLG2)属于 the bZIP家庭。 rTGA1、rTGA2和rTGA3与Arabidopsis TGA2， 分享75， 76和78%身分 相反， rLG2分享最高的身分(81%)对玉米liguleless (LG2)基因产品， which在建立介入叶子blade±sheath界限。 NPR1的互作用与米的nim1-4 削弱在酵母的bZIP蛋白质被npr1-1和npr1-2变化，但是不 NPR1±rTGA1互作用由一个体外下拉的实验con®rmed。 在胶凝体 mobility转移分析用试样， rTGA1困境对米RCH10促进者和对同边元素要求sequencespeci 柳酸快速响应的®cally。 这是那®rst的示范Arabidopsis NPR1 gene可能提高抗病力在monocot植物中。 这些结果也建议那monocot和 dicot种植份额控制NPR1斡旋的抵抗的一条被保存的信号转导路。
